Alpha-1000 SPECTROPHOTOMETER USER’S MANUAL V 1.0 Revision 0 Laxco, Inc. http://www.laxcoinc.com
10 The step-by-step procedure for making an absorption/transmittance measurement is as follows: 1. Turn on the spectrophotometer by pressing the po
11 blanking solution is placed into the sample compartment. If not, repeat the blanking process again. Lamp Changing 1. Remove any cuvettes from t
12 Figure-7 Bulb View of the Alpha-1000 Lamp Adjustment The position of your lamp has been factory adjusted to its optimal setting. Follow thes
13 4. Once the optimum position has been obtained, verify that the lamp chamber door is secure and all items are returned to their original state e
14 The transmittance varies from 0 to 100. A transmittance of 0%T represents complete absorption by the sample, whereas 100%T represents no absorpti
15 Experiment 2 Measurement of an Absorption Spectrum The absorption spectrum of a substance helps identify it. In this experiment, you will measu
16 The materials required for this experiment are 500 ml 0.04 g/l thymol blue solution, 40 ml 1 M HCL (aqueous), a 50 ml burette, a 10 ml graduated c
17 flasks, a 2 ml pipette, a 10 ml pipette, six 25 ml test tubes, six round or square cuvettes, and a hot plate. Procedure: 1. Fill the burette to
18 axis absorbance and mark it in equal intervals from 0 to a convenient round value above your highest data point. 2. Plot absorbance versus concen
19 6. Transfer the solution from the beaker to the 100 ml volumetric flask. Dilute the de-ionized water and mix thoroughly. This is your diluted e
20 Parts List Table-3 Alpha-1 Series Parts List Item Number Description A-1000 Model Alpha-1000 Spectrophotometer 15 nm Band pass Wavelength range
21 Troubleshooting PROBLEM Possible Cause Solution Instrument Inoperative (Power indicator has no light) Power cord not connected to outlet Plug i
22 PROBLEM Possible Cause Solution Instrument drift and noise No sufficient warm up time Significant temperature change Lamp not adjusted properly
3 Table of Contents Introduction ... 4 Wo
4 Introduction The Alpha-1000 is a single beam spectrophotometer, which is designed to meet the needs of both students and instructors. Its digital
Specifications Table-1 lists the specification for the Alpha-1000. Table-1 Wavelength Range 400~1000 nm Spectral Slit Width 15 nm Wavelength Accur
Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received eve
7 Wavelength Readout Window: Displays desired wavelength. USB port: It is used for connecting to Computer with Application Software RS232: It is u
8 Figure-3 Back view of Alpha-1000 Changing Sample Holders Your Alpha-1000 comes standard with the A-110-102 Sample Holder. We offer thre
9 Figure-4 Changing Sample Holders Figure-5 Allen Wrench Basic Operation Simple OPERATION INSTRUCTIONS are printed on the front panel of yo
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